Botanicare Hydroguard is a liquid root-zone additive — a beneficial-bacteria supplement you mix into your feed water and dose with regular waterings. This page gives the maker’s published formula, guaranteed analysis, and dose; explains what Botanicare says it does and how a Bacillus root inoculant works; shows the best uses, how it compares to other root-inoculant types, where it fits in your system, and how to store a living culture; and answers the common questions — all cited. It is a supplement that runs alongside your base nutrients, not a base nutrient itself.
What it is
Hydroguard is a liquid bacterial root inoculant. Botanicare positions it as a bacterial root inoculant designed to decompose organic matter and may convert it to nutrients that can be absorbed directly by plants’ roots. Its active organism is an isolated strain of Bacillus amyloliquefaciens, supplied as a liquid concentrate you dilute into the reservoir and add with every watering.
Guaranteed analysis and specifications
| Product type | liquid bacterial root inoculant (concentrate for reservoir dosing) |
|---|---|
| Active organism | Bacillus amyloliquefaciens |
| Guaranteed analysis | Bacillus amyloliquefaciens 0.038% |
| Minimum count | 1.00×10^4 colony-forming units per millilitre |
| Macronutrients (N-P-K) | none — a microbial inoculant, not a feeding nutrient |
| Application rate | 2 mL per gallon, added with every watering for the entire growth cycle |
| Directions | shake well before each use, never mix concentrates, and always allow nutrient solutions to mix well before adjusting pH |
| Sizes | 1-quart, 1-gallon, and 5-gallon bottles (this listing is the quart) |
Why choose Botanicare Hydroguard
- A named, single-organism inoculant. You know exactly what is in the bottle — an isolated strain of Bacillus amyloliquefaciens at a published guaranteed analysis of 0.038% and a stated minimum of 1.00×10^4 colony-forming units per millilitre.
- What Botanicare says it does. The maker states its beneficial bacteria rapidly colonize the root zone, and positions the product as one that may break down nutrients and make them readily available for the plant, so roots take up nutrients more easily as needed. These are Botanicare’s own positioning claims, not an independent test result.
- It won’t disturb your feed. Because it is a microbial product with no N-P-K, it adds negligible strength to your solution and will not meaningfully move your EC — so it runs alongside your base nutrients rather than changing the recipe.
- One simple rate for the whole cycle. There is no phase schedule to juggle: 2 mL per gallon, with every watering, the same in veg and bloom.
- Built for reservoir-fed growing. Its organism has been applied as a reservoir biofertilizer across deep-water and nutrient-film (NFT) hydroponic systems, and works at the root zone in soilless culture, which is exactly how you dose a liquid concentrate into a common reservoir.
- A living culture with a known mode. Root inoculants built on Bacillus bacteria — including Bacillus amyloliquefaciens and the closely related Bacillus subtilis, which colonize the root zone and suppress plant pathogens largely by producing lipopeptides (surfactin, iturin, and fengycin) and forming biofilms on the root surface — are well described in the horticultural literature, so you can understand the organism class independently of any marketing.
Best uses for Hydroguard
- Recirculating hydroponics (DWC and NFT). Bacillus amyloliquefaciens has been used across deep-water and nutrient-film (NFT) hydroponic systems, at the root zone, so it suits reservoir-fed recirculating setups run off a common tank.
- Drip and run-to-waste off a reservoir. Because Botanicare directs dosing with every watering during the entire growth cycle, it fits drip and hand-watered programs fed from the same nutrient solution.
- Soilless root zones (coco and peat mixes). The organism works at the root zone in soilless culture, so it is not limited to bare-root hydro.
- Alongside a full feed program. With no N-P-K of its own, it layers onto your existing base nutrients and additives without changing their strength.
- Warm reservoirs you are trying to keep in check. Root-zone Pythium turns aggressive above 77 F, so a reservoir supplement is most relevant when you are already working to hold temperatures down (see the operating range below).
How a Bacillus root inoculant works
Root inoculants built on Bacillus bacteria — including Bacillus amyloliquefaciens and the closely related Bacillus subtilis, which colonize the root zone and suppress plant pathogens largely by producing lipopeptides (surfactin, iturin, and fengycin) and forming biofilms on the root surface. That is a different mechanism from what the maker itself claims (breaking down organic matter), so this section describes the organism class generally, from the horticultural literature, not a Botanicare performance claim.
How it compares to other root inoculants
Root inoculants come in different organism classes. This table is factual — it does not claim any one out-performs another; which suits your program is a grower’s judgement.
| Factor | Botanicare Hydroguard | A Bacillus subtilis product | A Trichoderma product |
|---|---|---|---|
| Organism type | bacterium — Bacillus amyloliquefaciens | bacterium — Bacillus subtilis, closely related to B. amyloliquefaciens | fungus — Trichoderma, not a bacterium |
| Mode of action | lipopeptides (surfactin, iturin, fengycin) and root-surface biofilms | lipopeptides and root-surface biofilms — the same Bacillus mechanism class | mycoparasitism — attacking other fungi with cell-wall-degrading enzymes — plus competition and induced plant resistance |
| Form | liquid concentrate | varies by product | varies by product |
| Best fit | reservoir-fed hydro and soilless, dosed with every watering | grower’s choice among bacterial root inoculants | grower’s choice where a fungal inoculant is preferred |
Compatibility and operating range
Botanicare does not publish a product-specific pH window, temperature range, or storage instructions for Hydroguard, so the ranges below are clearly-labelled general horticultural guidance from university-extension and peer-reviewed sources — useful context for running a living inoculant, not a Botanicare specification.
- Systems: deep-water and nutrient-film (NFT) hydroponics, and soilless root zones — any setup dosed from a common reservoir.
- Solution pH: hydroponic nutrient solutions are typically held at pH 5.5 to 7.0.
- Reservoir temperature: keep it on the cool side, because root-zone Pythium turns aggressive above 77 F.
- Water source and dechlorination: chlorine and chloramine in municipal water are harmful to beneficial bacteria and should be removed by off-gassing or a dechlorinator (for chloramine, sodium thiosulfate or ascorbic acid / vitamin C) before you add a live culture.
- Sanitizer incompatibility: do not run the live culture alongside a reservoir chlorine sterilizer — the same chlorine that must be removed before use will work against the bacteria if it is dosed continuously.
- Tank-mix and feed strength: it carries no N-P-K, so it does not add nutrient salts to a tank mix or push your EC up.
- Storage: as a living culture, keep it cool and out of direct sunlight (a refrigerator is ideal; do not freeze), since heat and sunlight kill the bacteria, and use it before the expiration date.
Dosing and how to use it
Botanicare specifies a single rate for the entire growth cycle — 2 mL per gallon, added with every watering, the same in veg and bloom. There is no separate veg or bloom rate to mix. A simple order of operations:
- Start with dechlorinated water — off-gas it or use a dechlorinator, because chlorine and chloramine harm the beneficial bacteria.
- Add and mix your base nutrients first; always allow the nutrient solution to mix well before adjusting pH.
- Shake the bottle, then add Hydroguard at 2 mL per gallon, and never mix concentrates.
- Dose it with every watering during the entire growth cycle.
- Keep the reservoir cool (root-zone Pythium is aggressive above 77 F), and store the bottle cool and out of direct sunlight, never frozen.
Where it fits
For other options in this part of the catalogue, browse the roots additives category. General store policies are described on the about page. As a biological additive, what it does in your system depends on your routine and conditions.
Frequently asked questions
What is the active ingredient? Bacillus amyloliquefaciens at 0.038%, at a minimum of 1.00×10^4 CFU per millilitre.
How much and how often? 2 mL per gallon, with every watering through the whole growth cycle — one rate, no phase schedule.
Will it raise my EC or feed strength? No — it is a microbial product with no N-P-K, so it does not add nutrient salts.
Can I use it with tap water or a reservoir sterilizer? Dechlorinate first — chlorine and chloramine harm the beneficial bacteria and should be off-gassed or removed with a dechlorinator — and do not run it alongside a chlorine sterilizer.
Does it work in coco or soilless mixes, or only in water culture? Its organism works at the root zone in soilless culture as well as deep-water and NFT systems.
How do I store it? Keep it cool and out of direct sunlight, do not freeze, and use it before the expiration date.
Who this is for — and who should skip it
Suited: growers who want a beneficial-bacteria root-zone supplement to run alongside their normal feed schedule in a reservoir-fed hydroponic or soilless setup, and who are comfortable dosing with every watering rather than occasionally.
Not for / its limits: it is a supplement, not a base nutrient, so it does not feed a crop on its own. Botanicare’s own wording is hedged (“may convert,” “may break down”), and beyond the maker’s stated positioning we do not claim it prevents root rot, controls a named pathogen, out-performs another Bacillus or Trichoderma product, or builds “superior root mass.” Its organic-certification status is not listed on the maker’s page, so we assert none, and Botanicare publishes no product-specific pH window, temperature range, per-phase dose, or net weight — those are held rather than guessed. If you want a labelled disease remedy or a complete feeding program, this is not that product.
Ready to add it to your routine? Add to cart, or talk to a grower about whether a root inoculant fits your setup.
Sources
- Botanicare — Hydroguard product page (organism, guaranteed analysis, dose, sizes, directions, “decompose organic matter” positioning; retrieved 2026-07-01): https://www.botanicare.com/products/hydroguard/
- Botanicare — “How does Hydroguard work?” FAQ (“beneficial bacteria rapidly colonize the root zone”; retrieved 2026-07-02): https://www.botanicare.com/faqs/how-does-hydroguard-work/
- Botanicare — “What are the benefits of using Hydroguard?” FAQ (“may break down nutrients and make them readily available”; retrieved 2026-07-02): https://www.botanicare.com/faqs/what-are-the-benefits-of-using-hydroguard/
- Frontiers in Plant Science, 2024 — Bacillus amyloliquefaciens as a biofertilizer across deep-water and NFT hydroponic systems: https://pmc.ncbi.nlm.nih.gov/articles/PMC11456494/
- Frontiers in Cellular and Infection Microbiology, 2021 — Bacillus amyloliquefaciens lipopeptides and root-zone biocontrol: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253258/
- Trichoderma — Current Status of Its Application in Agriculture (peer-reviewed review, 2022; mycoparasitic mode of action): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875981/
- Penn State Extension — Inoculation of Forage and Grain Legumes (storing a living microbial inoculant): https://extension.psu.edu/inoculation-of-forage-and-grain-legumes
- Oklahoma State University Extension — Principles of Small-Scale Aquaponics (chlorine/chloramine harm beneficial bacteria; dechlorinate before use): https://extension.okstate.edu/fact-sheets/principles-of-small-scale-aquaponics.html
- UC Statewide IPM Program (UC ANR) — Pythium Root Rot (pathogen-active temperature): http://ipm.ucanr.edu/PMG/r280100211.html
- UNH Extension — Hydroponics at Home (general hydroponic solution pH range): https://extension.unh.edu/resource/hydroponics-home











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